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Image Search Results
Journal: Experimental Neurobiology
Article Title: M2 Phenotype Microglia-derived Cytokine Stimulates Proliferation and Neuronal Differentiation of Endogenous Stem Cells in Ischemic Brain
doi: 10.5607/en.2017.26.1.33
Figure Lengend Snippet: TGF-α is one of the major cytokines in M2 conditioned media. (A) RT-PCR of IL-10 and TGF-α in M0, M1, or M2 conditioned media. IL-10 and TGF-α expression is significantly increased at the mRNA level in M2 conditioned media. (B) The graph depicts the relative mRNA expression of IL-10 and TGF-α in M0, M1, and M2 conditioned media. The expression of these genes is significantly increased in M2 conditioned media. (C) The expression level of TGF-α in M0, M1, and M2 conditioned media using ELISA assay. The quantity of TGF-α is significantly increased in M2 conditioned media compared with M1 ( *** p<0.01 vs. M0 conditioned media, n=5/group).
Article Snippet: To determine the soluble TGF-α concentration in the different phenotypes of supernatant in BV2 cells, the conditioned media were measured by the
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay